Flow CytometryServices

Since the inception of this facility, there have been many requests for new services and new techniques have been added. The personnel and instrumentation available at the facility are capable of performing virtually any flow cytometric assay requested by IU Simon Cancer Center investigators.

Available instrumentation includes:

Analyzers:

  1. BD FACScan Flow Cytometer
  2. BD FACSCalibur Flow Cytometer
  3. BD LSRII Flow Cytometer

Cell Sorters:

  1. BD FACStar Plus Cell Sorter
  2. BD FACSVantage SE Cell Sorter
  3. BD FACSAria Cell Sorter

The address of BD (Becton Dickinson and Company) is:

BD Biosciences
2350 Qume Drive
San Jose, CA  95131-1807

Available services include:

  1. Multiparameter (4, 5 or 6-color) immunofluorescence analysis and cell sorting
  2. DNA content and cell cycle analysis
  3. Simultaneous immunofluorescence and cell cycle analysis
  4. Single cell sorting
  5. Cell sorting (bulk or single cells) based on position of cells in cell cycle
  6. Apoptosis analysis (Annexin V/PI, Caspase, TUNEL and other assays)
  7. BrdU incorporation
  8. Viability assays
  9. Chromosome analysis (univariate and bivariate)
  10. Receptor - ligand interactions
  11. Kinetic analyses
  12. Off-line data analysis