$105

Ficoll preparation of human blood MNCs

• Preparation includes overlaying of up to 100ml blood, washes and cell count

$60

CPT preparation of human blood MNCs

• Preparation includes processing of up to 48 ml of blood in CPT tubes supplied by the user, washes and cell count

$230

8 color FACS analysis of human blood MNCs

• Staining of MNCs with 7 antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.
• Does not include cost of data collection in Flow Cytometry Core
• Isolation of MNCs is a separate charge

$185

6 color FACS analysis of human blood MNCs

• Staining of MNCs with 6 antigen panel, including necessary single color and isotype controls, and brief analysis in FlowJo.
• Does not include cost of data collection in Flow Cytometry Core
• Isolation of MNCs is a separate charge

$105

CFU-EC quantitation from human blood

• Plating, culture and quantitation of CFU-ECs (in triplicate)
• Isolation of MNCs is a separate charge

$220

Quantitation of ECFCs from human blood

• Plating, maintanence, and quantitation of 3 wells for ECFC
• Isolation of MNCs is a separate charge

$125

Expansion and Cryopreservation of ECFCs

• Expansion of ECFC colonies to passage 2 and cryopreservation of cells

$600

Endothelial Cell Isolation from Human Umbilical Veins

• Isolation of ECs from human umbilical veins, expansion to passage 1 and phenotypic confirmation by FACS

$120

Brief Endothelial Phenotyping of ECs by FACS

• Staining of cell line with endothelial antigen panel to confirm phenotype including controls
• Does not include usage charge from Flow Cytometry Core

$265

Endothelial Progenitor Cell Clonogenic Assays

• Limiting dilution analysis of up to 6 conditions in triplicate

$305

Endothelial Progenitor Cell Clonogenic Assays

• Single cell analysis of up to 6 96 well plates
• Does not include sorting charge from Flow Cytometry Core
• Does not include cost of cell line

$300

Endothelial Cell Proliferation Assays (6 well format)

• Thymidine incorporation in 6 well plates for up to 6 conditions (including controls) in triplicate
• Does not include cost of a cell line

$210

Endothelial Cell Proliferation Assays (96 well format)

• Thymidine incorporation in 96 well plates for up to 10 conditions (including controls) with 6 replicates
• Does not include cost of cell line

$470

Endothelial Cell Migration Assays

• Haptotaxis in transwells for up to 8 conditions (including controls) in triplicate
• Does not include cost of cell line

$230

Tube Forming Assays with Human Endothelial Cells

• Up to 12 wells assayed for EPC tube formation

$310

Co-Culture Tube Forming Assays Using Transwell System

• Up to 12 wells assayed for EC tube formation
• Does not include cost of ECFC line

$540

Ex Vivo Aortic Outgrowth Model of Capillary Structures

• Aortic ring outggrowth using up to 6 mice (up to 5 conditions including controls)
• Does not include the cost of mice used

$250

Retroviral Transduction of Primary Human Endothelial Cells with Fluorescently Labeled Proteins

• Retroviral transduction with eGFP or dsRed and expansion of cell line

$215

Cryopreserved human UCB derived ECFC cell line

$215

Cryopreserved HUVEC cell line

Cost to be determined on an individual basis

Retroviral Transduction of Primary Human Endothelial Cells with short interference RNAs (siRNAs)

$445

Mouse Matrigel Plug Model

• Placement of 1-2 matrigel plugs in up to 6 mice, hemoglobin content analysis of plugs
• Does not include cost of mice or perdiems, which will be calculated and charged on an individual basis

$615

Transplantation of Human Endothelial Progenitor Cells into Immunodeficient Mice

• Placement of collagen/FN/EPC implants into up to 4 NOD/SCID or NS2 mice
• Does not include histological analysis of implants
• Does not include animal or perdiem costs

Cost to be determined on an individual basis

Bone Marrow Contribution to Tumor Microenvironments Utilizing Chimeric Mice with Green Fluorescent-Labeled Bone Marrow Cells